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ptwin1 vector  (New England Biolabs)


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    Structured Review

    New England Biolabs ptwin1 vector
    Ptwin1 Vector, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 218 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ptwin1/pTWIN1+Vector+DNA/pmc12032424-132-25-27
    Average 93 stars, based on 218 article reviews
    ptwin1 vector - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: Influence of RNA structural stability on the RNA chaperone activity of the Escherichia coli protein StpA
    Article Snippet: .. The plasmid used for cloning and purification of StpA and the respective mutant was pTWIN1 (no. N6951S) from the New England Biolabs IMPACTTM-TWIN System. ..

    Article Title: High-Yield Soluble Expression and Simple Purification of the Antimicrobial Peptide OG2 Using the Intein System in Escherichia coli
    Article Snippet: .. The two codon-optimized OG2 genes including C-OG2 (C-terminal fusion expression) gene and N-OG2 (N-terminal fusion expression) gene were amplified using splitting overlap extension (SOE) PCR and cloned into the pTWIN1 (NEB, USA) vector ( ). ..

    Cloning:

    Article Title: Influence of RNA structural stability on the RNA chaperone activity of the Escherichia coli protein StpA
    Article Snippet: .. The plasmid used for cloning and purification of StpA and the respective mutant was pTWIN1 (no. N6951S) from the New England Biolabs IMPACTTM-TWIN System. ..

    Purification:

    Article Title: Influence of RNA structural stability on the RNA chaperone activity of the Escherichia coli protein StpA
    Article Snippet: .. The plasmid used for cloning and purification of StpA and the respective mutant was pTWIN1 (no. N6951S) from the New England Biolabs IMPACTTM-TWIN System. ..

    Mutagenesis:

    Article Title: Influence of RNA structural stability on the RNA chaperone activity of the Escherichia coli protein StpA
    Article Snippet: .. The plasmid used for cloning and purification of StpA and the respective mutant was pTWIN1 (no. N6951S) from the New England Biolabs IMPACTTM-TWIN System. ..

    Polymerase Chain Reaction:

    Article Title: Synthesis of L-nucleic acids by means of an enzyme
    Article Snippet: .. PCR product 2 was made using pTWIN1 (New England Biolabs, Frankfurt am Main, Germany) as a template and primers MJ_1_72_DD (5′-Phosphate-AGCGGCTCTTCGTGCATCACGGGAGAT-3′, (SEQ ID: 66)) and MJ_1_73_DD (5′-Phosphate-AGCGGCTCTTCGCCCTTGAAGCTGCCACAAGGCAGGAACGTT-3′, (SEQ ID: 67)) to amplify the Mxe Gyr A intein and the CBD. .. PCR product 2 was made using pTWIN1 (New England Biolabs, Frankfurt am Main, Germany) as a template and primers MJ_1_72_DD (5′-Phosphate-AGCGGCTCTTCGTGCATCACGGGAGAT-3′, (SEQ ID: 66)) and MJ_1_73_DD (5′-Phosphate-AGCGGCTCTTCGCCCTTGAAGCTGCCACAAGGCAGGAACGTT-3′, (SEQ ID: 67)) to amplify the Mxe Gyr A intein and the CBD.

    Article Title: Synthesis of L-nucleic acids by means of an enzyme
    Article Snippet: .. PCR product 2 was made using pTWIN1 (New England Biolabs, Frankfurt am Main, Germany) as a template and primers MJ_1_72_DD (5′-Phosphate-AGCGGCTCTTCGTGCATCACGGGAGAT-3′, (SEQ ID NO: 66)) and MJ_1_73_DD (5′-Phosphate-AGCGGCTCTTCGCCCTTGAAGCTGCCACAAGGCAGGAACGTT-3′, (SEQ ID NO: 67)) to amplify the Mxe Gyr A intein and the CBD. .. PCR product 2 was made using pTWIN1 (New England Biolabs, Frankfurt am Main, Germany) as a template and primers MJ_1_72_DD (5′-Phosphate-AGCGGCTCTTCGTGCATCACGGGAGAT-3′, (SEQ ID NO: 66)) and MJ_1_73_DD (5′-Phosphate-AGCGGCTCTTCGCCCTTGAAGCTGCCACAAGGCAGGAACGTT-3′, (SEQ ID NO: 67)) to amplify the Mxe Gyr A intein and the CBD.

    Article Title: Adenosine Triphosphate Stimulates Aquifex aeolicus MutL Endonuclease Activity
    Article Snippet: The forward and reverse primers incorporated NdeI and SapI restriction sites, respectively, for subcloning into the pTWIN1 vector (New England Biolabs, Inc.). .. The PCR product was digested with NdeI and SapI restriction endonucleases (New England Biolabs, Inc) and subcloned into the same sites in pTWIN1 to create a direct fusion with the Mxe GyrA Intein-tag. .. Aae MutL-CTD and Aae MutL mutants E357K, H404A and R406A were generated using the PhusionTM Site-Directed Mutagenesis Kit (New England Biolabs, Inc.).

    Expressing:

    Article Title: High-Yield Soluble Expression and Simple Purification of the Antimicrobial Peptide OG2 Using the Intein System in Escherichia coli
    Article Snippet: .. The two codon-optimized OG2 genes including C-OG2 (C-terminal fusion expression) gene and N-OG2 (N-terminal fusion expression) gene were amplified using splitting overlap extension (SOE) PCR and cloned into the pTWIN1 (NEB, USA) vector ( ). ..

    Amplification:

    Article Title: High-Yield Soluble Expression and Simple Purification of the Antimicrobial Peptide OG2 Using the Intein System in Escherichia coli
    Article Snippet: .. The two codon-optimized OG2 genes including C-OG2 (C-terminal fusion expression) gene and N-OG2 (N-terminal fusion expression) gene were amplified using splitting overlap extension (SOE) PCR and cloned into the pTWIN1 (NEB, USA) vector ( ). ..

    Overlap Extension Polymerase Chain Reaction:

    Article Title: High-Yield Soluble Expression and Simple Purification of the Antimicrobial Peptide OG2 Using the Intein System in Escherichia coli
    Article Snippet: .. The two codon-optimized OG2 genes including C-OG2 (C-terminal fusion expression) gene and N-OG2 (N-terminal fusion expression) gene were amplified using splitting overlap extension (SOE) PCR and cloned into the pTWIN1 (NEB, USA) vector ( ). ..

    Clone Assay:

    Article Title: High-Yield Soluble Expression and Simple Purification of the Antimicrobial Peptide OG2 Using the Intein System in Escherichia coli
    Article Snippet: .. The two codon-optimized OG2 genes including C-OG2 (C-terminal fusion expression) gene and N-OG2 (N-terminal fusion expression) gene were amplified using splitting overlap extension (SOE) PCR and cloned into the pTWIN1 (NEB, USA) vector ( ). ..

    other:




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